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Curcumin treatment altered the expressions of mitochondrial proteins. Mitochondrial proteins including UCP2, <t>UCP3</t> and mtTFA were determined by the western blotting assay. The COX IV was used as the internal control. (A) The blots of UCP2, UCP3 and mtTFA. (B) The relative expression of the protein level of UCP2. (C) The relative expression of the protein level of UCP3. (D) The relative expression of the protein level of mtTFA Cur: curcumin; Con: control; UCP2: mitochondrial uncoupling protein 2; UCP3: mitochondrial uncoupling protein 3; mtTFA: mitochondrial transcription factor A; * p < 0.05 vs. the Sham; # p < 0.05 vs. the Con.
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Curcumin treatment altered the expressions of mitochondrial proteins. Mitochondrial proteins including UCP2, <t>UCP3</t> and mtTFA were determined by the western blotting assay. The COX IV was used as the internal control. (A) The blots of UCP2, UCP3 and mtTFA. (B) The relative expression of the protein level of UCP2. (C) The relative expression of the protein level of UCP3. (D) The relative expression of the protein level of mtTFA Cur: curcumin; Con: control; UCP2: mitochondrial uncoupling protein 2; UCP3: mitochondrial uncoupling protein 3; mtTFA: mitochondrial transcription factor A; * p < 0.05 vs. the Sham; # p < 0.05 vs. the Con.
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Curcumin treatment altered the expressions of mitochondrial proteins. Mitochondrial proteins including UCP2, <t>UCP3</t> and mtTFA were determined by the western blotting assay. The COX IV was used as the internal control. (A) The blots of UCP2, UCP3 and mtTFA. (B) The relative expression of the protein level of UCP2. (C) The relative expression of the protein level of UCP3. (D) The relative expression of the protein level of mtTFA Cur: curcumin; Con: control; UCP2: mitochondrial uncoupling protein 2; UCP3: mitochondrial uncoupling protein 3; mtTFA: mitochondrial transcription factor A; * p < 0.05 vs. the Sham; # p < 0.05 vs. the Con.
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Curcumin treatment altered the expressions of mitochondrial proteins. Mitochondrial proteins including UCP2, <t>UCP3</t> and mtTFA were determined by the western blotting assay. The COX IV was used as the internal control. (A) The blots of UCP2, UCP3 and mtTFA. (B) The relative expression of the protein level of UCP2. (C) The relative expression of the protein level of UCP3. (D) The relative expression of the protein level of mtTFA Cur: curcumin; Con: control; UCP2: mitochondrial uncoupling protein 2; UCP3: mitochondrial uncoupling protein 3; mtTFA: mitochondrial transcription factor A; * p < 0.05 vs. the Sham; # p < 0.05 vs. the Con.
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anti-ucp3 rabbit polyclonal antibody  (Thermo Fisher)
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Curcumin treatment altered the expressions of mitochondrial proteins. Mitochondrial proteins including UCP2, <t>UCP3</t> and mtTFA were determined by the western blotting assay. The COX IV was used as the internal control. (A) The blots of UCP2, UCP3 and mtTFA. (B) The relative expression of the protein level of UCP2. (C) The relative expression of the protein level of UCP3. (D) The relative expression of the protein level of mtTFA Cur: curcumin; Con: control; UCP2: mitochondrial uncoupling protein 2; UCP3: mitochondrial uncoupling protein 3; mtTFA: mitochondrial transcription factor A; * p < 0.05 vs. the Sham; # p < 0.05 vs. the Con.
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Curcumin treatment altered the expressions of mitochondrial proteins. Mitochondrial proteins including UCP2, UCP3 and mtTFA were determined by the western blotting assay. The COX IV was used as the internal control. (A) The blots of UCP2, UCP3 and mtTFA. (B) The relative expression of the protein level of UCP2. (C) The relative expression of the protein level of UCP3. (D) The relative expression of the protein level of mtTFA Cur: curcumin; Con: control; UCP2: mitochondrial uncoupling protein 2; UCP3: mitochondrial uncoupling protein 3; mtTFA: mitochondrial transcription factor A; * p < 0.05 vs. the Sham; # p < 0.05 vs. the Con.

Journal: Frontiers in Cardiovascular Medicine

Article Title: Curcumin Improves Cardiopulmonary Resuscitation Outcomes by Modulating Mitochondrial Metabolism and Apoptosis in a Rat Model of Cardiac Arrest

doi: 10.3389/fcvm.2022.908755

Figure Lengend Snippet: Curcumin treatment altered the expressions of mitochondrial proteins. Mitochondrial proteins including UCP2, UCP3 and mtTFA were determined by the western blotting assay. The COX IV was used as the internal control. (A) The blots of UCP2, UCP3 and mtTFA. (B) The relative expression of the protein level of UCP2. (C) The relative expression of the protein level of UCP3. (D) The relative expression of the protein level of mtTFA Cur: curcumin; Con: control; UCP2: mitochondrial uncoupling protein 2; UCP3: mitochondrial uncoupling protein 3; mtTFA: mitochondrial transcription factor A; * p < 0.05 vs. the Sham; # p < 0.05 vs. the Con.

Article Snippet: The following antibodies were used for western blotting analysis: rabbit monoclonal anti-uncoupling protein 2 (UCP2) (#89326, Cell Signaling Technology, United States; 1:1,000 dilution), rabbit polyclonal anti-uncoupling protein 3 (UCP3) (#BA1722, Boster Biological Technology, United States; 1:500 dilution), rabbit polyclonal anti-cytochrome C (Cyt C) (#ab90529, Abcam, United Kingdom; 1:1,000 dilution), rabbit monoclonal anti-mitochondrial transcription factor A (mtTFA) (#ab252432, Abcam, United Kingdom; 1:1,000 dilution), rabbit monoclonal anti-Cyt C oxidase subunit IV (COX IV) (#ab202554, Abcam, United Kingdom; 1:2,000 dilution), rabbit monoclonal anti-caspase 3 (#ab184787, Abcam, United Kingdom; 1:5,000 dilution), rabbit polyclonal anti-cleaved-caspase 3 (#9661, Cell Signaling Technology, United States; 1:1,000 dilution).

Techniques: Western Blot, Control, Expressing